Asian Journal of Research in Chemistry

Somsubhra Ghosh ¹, B. Anusha ¹, Santhoshi ¹, David Banji ¹, Y. Chaithanya Kumar ¹, P. Raghavendra ¹, Subhadip Roy ²

Affiliations
1 Nalanda College of Pharmacy, Nalgonda, Andhra Pradesh, 508001, IN
2 Mylan Laboratories Ltd, Hyderabad, Andhra Pradesh, IN

Abstract

A new simple, accurate, rapid and precise isocratic High Performance Liquid Chromatographic (HPLC) method was developed and validated for the determination of Aliskiren Hemifumarate (ALSK) and Valsartan (VAL) in bulk drug. The Method employs Waters HPLC system on C8 Column (4.6 x 250 mm, 5 μm) and flow rate of 1 ml/min with a load of 10μl. The Detection was carried out at 220 nm. mobile phase used as Acetonitrile and Phosphate buffer and Methanol was used as mobile phase in the composition of 45:40:15 , phosphate buffer (0.02Mm) adjusted the pH to 4 with Orthophosphoric acid within a short runtime of 8 min. The retention times of Aliskiren (ALSK) was 3.407 min, Valsartan (VAL) was 4.268 min. The method was validated according to the regulatory guidelines with respect to specificity, precision, accuracy, linearity and robustness etc.

Keywords

Aliskiren Hemifumarate, Valsartan, HPLC, Validation

Full Text

     

Dipanjan Karati ¹, Somsubhra Ghosh ², Parna Pati ³, Debgopal Ganguli ⁴, Puja Saha ⁵

Affiliations
1 School of Pharmacy, Dept. of Pharmaceutical Science, Techno India, Kolkata, W. B., India, 700091 ., IN
2 School of Pharmacy, The Neotia University, Jhinger Pole, Dimond Harbour, W.B., India, 743368., IN
3 Department of Pharmaceutics, DmbH Institute of Medical Science, Dadpur, Hooghly, W. B., India, 712305., IN
4 School of Pha .rmacy, Seacom Skills University, Bolepur, W. B., India, 713125, IN
5 Mata Gujri College of Pharmacy, Mata Gujri University, Kishanganj, Bihar, India, 855107 ., IN

Abstract

Chromatography is a method for the isolation of a mixture into separate sections. It is usually based on the principle of partition of solute between two phases. It usually consists of a mobile phase and a stationary phase. The advancement of the mobile process in the planar stationary stage is evoked by different external or internal forces. Capillarity and gravity are the intrinsic force whereas external forces are electric field, pump, and centrifugal forces. Thin layer chromatography and high-pressure thin layer chromatography are based on the adsorption theory. Forced flow thin layer chromatography is also based on the adsorption principle. FFTLC is an authoritative method of separation that uses a solvent pump to extract the sample mixture into a pressurized ultra-micro cavity containing an adsorbent analytical or preparative planer bed. FFTLC needs no or slight sample preparation as in TLC. One relation between FFTLC and high-pressure liquid chromatography is that as in HPLC, the maximum layer length is used for analytical and preparatory separation. FFTLC is a process in which continuous production and continual evaporation of the mobile phase from the end of the chromatography plate produces forced-flow. Here, we focus on FFTLC, an advanced and more sophisticated chromatographic technique.

Keywords

Forced flow thin layer chromatography, High performance thin layer chromatography, Thin layer chromatography, High performance liquid chromatography .

Full Text